I updated my ctffind4 installation from veriosn 4.1.4 to version 4.1.13. Using the same parameters as before the update, I now get worse defocus estimations.
Details: I am estimating the defocus of a tomographic tilt series. Each tilt image was drift corrected but not dose-weighted. The target defocus was 3.5um. Around small tilt-angles the estimation seems ok. At higher tilt angles the 4.1.13 version is completely off while the 4.1.4 version gives good results. I am using the same parameters that gave good results on many other tilt series. You can find details about the chosen parameters and a plot of the defocus in the attached image (the tilt numbers 1-41 in the plots represent the tilts from -60 degrees to +60).
Am I making a mistake in the parameter selection or is this maybe a bug?
Thanks a lot for your help,