CtfFind4.1 loading mrc file error

Submitted by dgirodat on Thu, 2020-06-11 17:49

Forums

ctffind & ctftilt

I am a new user to relion and CtfFind and I've been encountering an issue with CtfFind on a set of mrc files. When I try to use CtfFind in Relion with these mrc files I receive the error:

WARNING: skipping, since cannot get CTF values for

Did not find any of the expected EPS files to generate a PDF file

+Will make an empty PDF-file in CtfFind/job002/logfile.pdf

When I look at the log file for CtfFind it says:

Error: File does not exist, please provide an existing file!

I decided to go around Relion and load these files into CtfFind on their own however, I receive the error:

File type: MRC

Dimensions: X = 5120 Y = 3840 Z = 1

Number of slices: 1

*** Caught unhandled unknown exception; terminatin

 

It appears something is wrong with either my mrc files or my CtfFind, any ideas.

Thank you in advance,

Dylan Girodat

Alexis Thu, 2020-06-11 19:53

Hi Dylan,

Thanks for trying ctffind. Could you please attach one of the MRC files that cause this error, so that I can investigate?

Thanks
Alexis

dgirodat Fri, 2020-06-12 11:17

Hi Alexis,

Thank you for the help. Forgive me but I am having difficulty finding how to attach an MRC file to the forum. Should I email one to you?

Thanks again,

Dylan