CTF determination from image stack

Submitted by Unknown on
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ctffind & ctftilt

I'm trying to figure out whether CTFFIND3 is able to estimate the CTF for a stack of images rather than for a single large image. After looking through the paper on CTFFIND3 and the Fortran code, my conclusion is that the program will not use a stack of images. Is this correct? Do you have a program that will use a stack of images to estimate CTF?

niko Tue, 2013-04-16 13:02

You are correct. However, you could montage all your images into one big image, then use CTFFIND3 with a box size that is the same as that of your original particle images. CTFFIND3 will then re-box all the original images and perform a CTF fit.

Alexis Tue, 2015-06-02 21:35

Hello Dong-Hua,

If your goal is to estimate the CTF from a stack of images (for example, frames of a movie), you could simply use ctffind4, which accepts stacks as input.

Otherwise, I'm not sure what the easiest way to montage images is, but I would begin by looking at IMOD, EMAN2 etc to see if there is a program for this purpose.

Alexis