Year of Publication
J Struct Biol
Cryoelectron Microscopy/*methods, "Image Processing, Computer-Assisted/*methods", Tobacco Mosaic Virus/ultrastructure
A low beam intensity, low electron dose imaging method has been developed for single-particle electron cryo-microscopy (cryo-EM). Experiments indicate that the new technique can reduce beam-induced specimen movement and secondary radiolytic effects, such as "bubbling". The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution.