The
modular functions of Signature
are grouped into various control panels (OpPanel). An OpPanel can be
activated by one of the OpMode buttons
located at the bottom of the main display window.
Micrograph
OpPanel
This
panel provides functions for micrograph inspection and micrograph
masking. When a new micrograph is loaded, the system will be
automatically switched to Micrograph
OpPanel, in which the user can
- Set the pixel resolution of the micrograph (in Å)
- Examine image quality (histogram and power spectrum)
- Apply automatic and manual micrograph masking
- Record a brief message about the image at Keynote
- Adjust image brightness and contrast for better
visualization
Template
OpPanel
This
panel provides functions for template image masking and search
parameter setup. Note that both X- and
Y-dimensions of
each template image are required to have at least 32 pixels. When
a new template stack is loaded, the system will be automatically
switched to Template
OpPanel, in which the user can
- Set masking radius for LCF screening (by percentage)
- Set masking radii (inner and outer) for SCF screening
- Apply manual masking tools to customize mask shape
for each frame
- Activate the level-II screening, if necessary, and build
corresponding masks
- Set the range and step size of rotational search (in
degrees)
Particle
OpPanel
This
panel provides functions for algorithmic/manual particle screening,
selection and inspection. When
working in Particle
OpPanel, the user can
- Define the approximate particle diameter (Size,
in Å)
- Set the micrograph Resize
factor
- Set LCF
/ SCF
thresholds and intra-particle separation (Dist)
restraint for screening. The separation distance is between particle
boundaries
- Apply test screening (
) to
ensure correct parameter setup (optional)
- Apply automatic full-range particle screening (Screening)
- Apply manual particle selection
- Inspect the particle selection through monitors
- Build particle stack and send it to Image
Stack Editor for
further processing
2D Image OpPanel
This
panel provides functions for image cropping (include filament boxing)
and image processing. When
working in 2D-Image
OpPanel, the user can
- Crop a rectangular image section from the micrograph
- Box a filement along any direction
- Apply various 2D image processing functions
3D Model OpPanel
This panel provides functions for 3D density map display and image
processing. When working in 3D-Model
OpPanel, the user can
- Load and display a 3D density map
- Examine density distribution by level-set display
- Generate 2D projections at regular angular intervals
- Apply various 3D image processing functions
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