This
module provides functions for particle-mass histogram display and
sorting. After proper intensity normalization, the particle mass can be
readily estimated from the optical density of the image. Particles of
the same type should reside closely in the mass histogram. This
function can therefore be used to
- separate heterogeneous particles with distinctive mass
difference;
- scrutinize particle selection by eliminating extreme cases.
To apply this function, particle images should first be loaded from Image
Stack Editor. Then switch to this functional panel at Menu
: Function : Digital Gel Filtration, set Masking
Radius in the Mass
Distribution box to match the particle size. Click the Apply
button to generate the mass distribution statistics. Then set the
lower-band limit (the red bar) and higher-band limit (the green bar)
accordingly. The values can be modified either through the input boxes
in Threshold
Parameters or by mouse operation - position the mouse cursor
close to the left of the red bar, click and drag, or position the mouse
cursor close to the right of the green bar, click and drag. Once the
threshold setup is ready, click Apply
to count the particles inside the band.
Both the masking radius and the band-limits can be repeatedly modified.
When a satisfactory result has been reached, click Commit
Selection to finalize the operation. The Image
Stack Editor will show up with selected image frames marked.
These images can be extracted and saved to a new stack. |