- Load a particle stack "particles.mrc"
into Image
Stack Editor.
- Browse the image stack with arrow-buttons in the Display
Parameters box.
- Image filtering: set band-limits, click the Filter
button in Image
Processing box.
- Image normalization: set the mean and s.t.d. values, click
the Norm
button.
- Invert intensity contrast by setting Multiplier
to –1 and click the Scale
button.
- Image frame cropping and padding with the Crop
function.
- Image frame selection: position the mouse cursor inside the
image frame, press CTRL and left-click
- Image frame Extract
and Delete:
applicable to the selected frames.
- Switch to Menu
: Function : Digital Gel Filtration. Set Masking
Radius to 0.75 and click Apply
in Mass
Distribution box to generate the density mass statistics.
- Use mouse to drag the red and green bars in the histogram
display to define an appropriate band of usable particles. Then click Apply
in Threshold
Parameters box. The number of selected particles will be
counted. Press Commit
Selection to accept the selection. The Image
Stack Editor will show up again, with selected particles marked
in blue frames. Accept the selection by clicking the Extract
button. A new image stack will be formed.
- Browsing through the resulting image stack. Should some
particle be removed, highlight it by pressing CTRL and left-click. At
the end, apply the Delete
button to remove those selected frames. Save the images
by clicking the Save
button to finalize the particle quality inspection.
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