Using the F200 for Absorbance end-point determinations
1. Combine samples and assay components in a microplate as per protocol.
2. Turn on the Tecan F200 and open the i-Control software.
3. In the menu select your microplate. The Grigorieff lab uses Greiner 96 well flat transparent plates (GRE96ft). Once you have selected a plate, you can highlight sections or individual wells to be read. 

4. Moving down the left hand side menu, after the microplate is named and configured the next step is to select the wavelength for the absorbance to be read at. Since this is a filter-based microplate, you are limited to the filters that are in the F200. If you have questions or need other filters, talk to Mike.

5. If you wish to use the temperature control, shaking, or move the plate in and out, set these values. Note that you can also use the top icon bar to insert the plate. 

6. Run the test using the icon bar. The data will dump automatically into an Excel spreadsheet for further processing.